Short answer, a mash-out should be enough. Just hitting a boil is more than enough.
Mashing-out in this case would be key. You'll want to stop all enzymatic activity anyway, so that your wort is stable overnight. This consists of holding the wort at 170°F(76°C) for ten minutes. Here's a pasteurization curve for milk.
![alt text](https://i.stack.imgur.com/uKeFR.gif)
(source: nzifst.org.nz)
On the UK standard, an additional 5°C equates to one order of magnitude reduction in the time necessary to kill. That is, a pathogen that's killed at 69°C in one minute will die at 64°C in ten minutes.
HTST (high-temperature, short time) pasteurization for milk is conducted at 161°F(71°C) for 15-20 seconds. Mashing out happens at 170°F(76°C) for ten minutes. HTST pasteurization acheives a 5-log, or 99.999% reduction in pathogens. By this math, in the first 15 seconds of your mash-out, you're killing ten times as many pathogens as milk pasteurization. If you extend your mash-out to fifteen minutes, you're applying 100 times the thermal killing power that we do to milk. (It should be said, though, that the point of milk pasteurization is to kill pathogens that might harm humans, not to kill bacteria that might contribute to off flavors. But a hundredfold increase is worth something.)
If you want to get in to the science of it, here's a paper on beer pasteurization, which gets into the survival of various bacteria, including Lactobacillus delbruckii.
People want something easy to remember. "Heat it to boiling and you're fine." But killing micro-organisms happens over a time-temperature curve, not at a particular temperature. That's why chefs who use the sous vide method of cooking are able to hold highly spoilage-prone materials like beef short rib at a pathogen-friendly 130°F for 36 hours(!) with no ill effects. This kind of cooking gives the health inspectors the howling fantods, but it's not some sort of home-grown wisdom. It's science.
Short answer, a mash-out should be enough. Just hitting a boil is more than enough. In cooling down, you might generate some DMS, but it will be driven off when you re-boil it later.