I am considering making some yeast slants to try to start a little bit of a yeast bank. In my research i have read about folks making a starter from the yeast in the bottom of a bottle conditioned beer, then inoculating the slants from that. I have also read about washing the yeast cake, but cant seem to find if there is another step between washing the yeast and inoculating slants. Is it better to just wait and use …

I've been looking into making yeast slants, thinking it could be fun. I've been looking for some test tubes online, and have a few questions. Is it tough to use test tubes with stoppers rather than screw on caps? Will I be able to properly sanitize polyethylene, or should I spend the extra few bucks on glass? Where is a good place to get them? Are petri dishes better than test tubes?